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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant function in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two extra splice variants had been noted but thorough nucleotide sequence information is not nonetheless available.
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Blocking peptides are peptides that bind especially on the target antibody and block antibody binding. These peptide usually includes the epitope regarded through the antibody.
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This manual outlines further feasible optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...
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This guide outlines more attainable optimization of mobile and immunoassay parameters to make sure the best possible benefits are...
Antibodies bound to the blocking peptide not bind for the epitope around the target protein. By evaluating the staining through the blocked antibody vs . the antibody by itself, you can see which staining is precise.
Focus on Facts Cyclin E belongs towards the extremely conserved cyclin loved ones, whose check here customers are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation designs which lead to your temporal coordination of each and every mitotic event. Cyclin E kinds a fancy with and features being a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and it is degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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